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ecto domain  (Rockland Immunochemicals)


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    Rockland Immunochemicals ecto domain
    Development and validation of <t>novel</t> <t>monoclonal</t> antibodies to the <t>ecto</t> domain of TrIP. A , cloning strategy and development of the ecto-TrIP Fc-fusion protein used for immunization and antibody development. B , SDS-PAGE of the ectoTrIP-Ig fusion protein under reducing and nonreducing conditions. C , flow cytometry data showing the binding of each anti-TrIP monoclonal antibody to a Flag-tagged WT murine TrIP construct expressed in HEK293T cells. D , flow cytometry data showing staining of the clones on HEK293T cells transfected with a plasmid encoding Flag-tagged human TrIP. E , flow cytometry data showing the staining of each of the anti-TrIP mAbs on HEK293T cells transfected with a Flag-tagged mTrIP construct lacking the extracellular kringle domain (ΔKringle). HEK, human embryonic kidney; mAb, monoclonal antibody; mTrIP, murine TrIP; TrIP, transmembrane inhibitor of PI3K.
    Ecto Domain, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecto+domain/pmc11615590-302-4-17?v=Rockland+Immunochemicals
    Average 92 stars, based on 1 article reviews
    ecto domain - by Bioz Stars, 2026-08
    92/100 stars

    Images

    1) Product Images from "Downregulation of PIK3IP1/TrIP on T cells is controlled by TCR signal strength, PKC, and metalloprotease-mediated cleavage"

    Article Title: Downregulation of PIK3IP1/TrIP on T cells is controlled by TCR signal strength, PKC, and metalloprotease-mediated cleavage

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2024.107930

    Development and validation of novel monoclonal antibodies to the ecto domain of TrIP. A , cloning strategy and development of the ecto-TrIP Fc-fusion protein used for immunization and antibody development. B , SDS-PAGE of the ectoTrIP-Ig fusion protein under reducing and nonreducing conditions. C , flow cytometry data showing the binding of each anti-TrIP monoclonal antibody to a Flag-tagged WT murine TrIP construct expressed in HEK293T cells. D , flow cytometry data showing staining of the clones on HEK293T cells transfected with a plasmid encoding Flag-tagged human TrIP. E , flow cytometry data showing the staining of each of the anti-TrIP mAbs on HEK293T cells transfected with a Flag-tagged mTrIP construct lacking the extracellular kringle domain (ΔKringle). HEK, human embryonic kidney; mAb, monoclonal antibody; mTrIP, murine TrIP; TrIP, transmembrane inhibitor of PI3K.
    Figure Legend Snippet: Development and validation of novel monoclonal antibodies to the ecto domain of TrIP. A , cloning strategy and development of the ecto-TrIP Fc-fusion protein used for immunization and antibody development. B , SDS-PAGE of the ectoTrIP-Ig fusion protein under reducing and nonreducing conditions. C , flow cytometry data showing the binding of each anti-TrIP monoclonal antibody to a Flag-tagged WT murine TrIP construct expressed in HEK293T cells. D , flow cytometry data showing staining of the clones on HEK293T cells transfected with a plasmid encoding Flag-tagged human TrIP. E , flow cytometry data showing the staining of each of the anti-TrIP mAbs on HEK293T cells transfected with a Flag-tagged mTrIP construct lacking the extracellular kringle domain (ΔKringle). HEK, human embryonic kidney; mAb, monoclonal antibody; mTrIP, murine TrIP; TrIP, transmembrane inhibitor of PI3K.

    Techniques Used: Biomarker Discovery, Bioprocessing, Cloning, SDS Page, Flow Cytometry, Binding Assay, Construct, Staining, Clone Assay, Transfection, Plasmid Preparation



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    Image Search Results


    Development and validation of novel monoclonal antibodies to the ecto domain of TrIP. A , cloning strategy and development of the ecto-TrIP Fc-fusion protein used for immunization and antibody development. B , SDS-PAGE of the ectoTrIP-Ig fusion protein under reducing and nonreducing conditions. C , flow cytometry data showing the binding of each anti-TrIP monoclonal antibody to a Flag-tagged WT murine TrIP construct expressed in HEK293T cells. D , flow cytometry data showing staining of the clones on HEK293T cells transfected with a plasmid encoding Flag-tagged human TrIP. E , flow cytometry data showing the staining of each of the anti-TrIP mAbs on HEK293T cells transfected with a Flag-tagged mTrIP construct lacking the extracellular kringle domain (ΔKringle). HEK, human embryonic kidney; mAb, monoclonal antibody; mTrIP, murine TrIP; TrIP, transmembrane inhibitor of PI3K.

    Journal: The Journal of Biological Chemistry

    Article Title: Downregulation of PIK3IP1/TrIP on T cells is controlled by TCR signal strength, PKC, and metalloprotease-mediated cleavage

    doi: 10.1016/j.jbc.2024.107930

    Figure Lengend Snippet: Development and validation of novel monoclonal antibodies to the ecto domain of TrIP. A , cloning strategy and development of the ecto-TrIP Fc-fusion protein used for immunization and antibody development. B , SDS-PAGE of the ectoTrIP-Ig fusion protein under reducing and nonreducing conditions. C , flow cytometry data showing the binding of each anti-TrIP monoclonal antibody to a Flag-tagged WT murine TrIP construct expressed in HEK293T cells. D , flow cytometry data showing staining of the clones on HEK293T cells transfected with a plasmid encoding Flag-tagged human TrIP. E , flow cytometry data showing the staining of each of the anti-TrIP mAbs on HEK293T cells transfected with a Flag-tagged mTrIP construct lacking the extracellular kringle domain (ΔKringle). HEK, human embryonic kidney; mAb, monoclonal antibody; mTrIP, murine TrIP; TrIP, transmembrane inhibitor of PI3K.

    Article Snippet: Monoclonal antibodies to the ecto domain of murine TrIP were developed in conjunction with a commercial vendor (Rockland, Inc).

    Techniques: Biomarker Discovery, Bioprocessing, Cloning, SDS Page, Flow Cytometry, Binding Assay, Construct, Staining, Clone Assay, Transfection, Plasmid Preparation